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To be clear, I do not believe CBD is a magic wand that made my IVF successful
Our data place redox regulation at the centre of this cascade: BzATP stimulation lowers cytosolic iGSH while increasing eGSH, and the magnitude of exosome release positively tracks this GSH efflux
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Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest
