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a degraded vial will not perform no matter how carefully it is dosed
CFU numbers were corrected for if any dilutions were made to the supernatant or lysates before plating
Plant Physiol Biochem 48(8):663672 Chen P, Ran S, Li R, Huang Z, Qian J, Yu M, Zhou R (2014) Transcriptome de novo assembly and differentially expressed genes related to cytoplasmic male sterility in kenaf ( Hibiscus cannabinus L .)
If this is the case, it could be that you have a Vitamin B-12 deficiency, and although there is no magic cure, the fix might just be a simple one
