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doi: 10.1186/s12989-023-00556-4

Although the alveolar space is exposed to the oxidants present in cigarette smoke, the alveolar glutathione pool is increased by 80% with no significant oxidant stress, as evidenced by the minimal shift toward the oxidized moiety oxidized glutathione (GSSG)

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Furthermore, methylene blue induced a dose and time dependent generation of superoxide anion from rabbit pulmonary arterial smooth muscle cells, as evidenced from spectrophotometric determination of cytochrome c reduction