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Additionally, in 2D cultures, ECs can easily spread and migrate due to the lack of physical impediments, while in 3D cultures, a surrounding matrix poses constraints that enforce the ECs adaptation to fit through matrix pores and often require matrix degradation for spreading and migration [240]

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The reaction was initiated by adding 4 L of 25 M stock purified NNMT protein (final concentration of NNMT in the reaction was 200 nM) and incubated on a heat block at 37 C