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Description
Western immunoblot analysis The serum samples and liver homogenates (previously prepared for determination of oxidative stress biomarkers) were separated by 12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Hybond-P, Amersham Pharmacia Biotech), blocked in a 0.2% Tween 20, 50 mM Tris HCl pH 7.6, and 150 mM NaCl solution containing 5% non-fat condensed milk
Hawkins, D.R., Cheng, K.N., Major, R.M., (1994)
PMID: 8758120
Acyl CoA transforms into acetyl CoA through -oxidation
