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Aged oocytes showed increased H2AX fluorescence intensity relative to fresh controls, indicating substantial DNA damage

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In mouse embryonic fibroblasts (MEFs) generated from IALP-knockout mice, the protein expression levels of zolunin-1 (ZO-1), zonulin-2 (ZO-2), and occludin were significantly lower compared to the levels of these proteins in wild-type control cells [28]