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PBS was used to wash the sections three times, with each wash lasting 5 min
doi: 10.1016/j.freeradbiomed.2021.02.010 39
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Similarly, a study based on swimming exercise for a total of four hours showed an increase in NAD + levels, yet also in this case, no measurements of mitochondrial function were performed 309 , reinforcing the need for more exercise-based investigations to further define how NAD + and mitochondrial homeostasis are perturbed and connected during exercise
