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Description
For microscope observation, after incubation, the cells climbing washed twice with JC-1 staining buffer, nuclear staining with Hoechst 33,342 images were captured using a confocal microscope (SP8, Leica)
If you're only using one squirt and still getting some reactions, just take a few drops of it and slowly, slowly increase it over like seven or eight days
The distinction matters because vasodilation is temporary, requiring continuous application to maintain
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