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For example, cultured astrocytes subjected to oxygen and glucose deprivation showed a decrease in mitochondrial membrane potential, possibly caused by the mitochondrial permeability transition pore (mtPTP) opening, which leads to a loss of intramitochondrial contents, mitochondrial respiration and ATP production [48]

Though the mechanism for DMF function is still not fully understood, published reports support the therapeutic potential of DMF in oxidative stress regulation in alleviating SCD symptoms

Cell immunofluorescence staining For cell immunofluorescence microscopy, cells were seeded on 12 mm coverslips and washing by ice-cold (PBS, pH = 7.4)

Mengfa et al