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Beads were washed four times with NT2 buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM MgCl 2 , 0.05% NP-40) and RNA extracted and quantified as previously described 76,115,116

Such a strategy was also adopted by the authors of the previously cited various vitamin B12 desensitization protocols [9,10,11,12,13,14,15,16]

2 O 2 but also regenerates two critical antioxidants, AsA and GSH, aiding in the maintenance of reactive oxygen species (ROS) and redox balance in plants ( Figure 3 )

SH3 domain is required for membrane bulge formation In contrast to the assembly at the CCP, the SH3 domain was required to form the membrane bulge