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Description
10.1093/tropej/45.5.294

Insert needle at a 45 angle (or 90 if using very short needle and ample subcutaneous tissue) into the subcutaneous fat layer [11] [12]

It is noteworthy that patients or their legal guardians, all investigators, and data analysts were blinded

SPCD is inherited in an autosomal recessive manner, with mutated alleles coming from both parents
