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Description
The assay plate was incubated for 3 h at 37 C in 5% CO 2 and allowed to equilibrate at room temperature for 5 min after which 30 L SteadyLite Plus (PerkinElmer, 6066759) was added to each well

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For an extra boost, you can add a CoQ10 injection for mitochondrial support, Glutathione for antioxidant protection, or B12 for mood and mental clarity

10.2337/db09-1918 51 GranadoM.Fuente-MartinE.Garcia-CaceresC.ArgenteJ.ChowenJ
