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When a potential is applied to the electrodes, redox reactions occur, leading to a measurable electrical signal, which changes in current or potential corresponding to the analytes concentration (OBrien et al., 2021)

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Acknowledgments The author thanks the Faculty of Medicine, Chiang Mai University, for academic support and access to institutional resources

Briefly, the peptide samples were trapped and washed on Pepmap100 C 18 trap (5m, 0.3 5 mm) at 20ul/min using 2% acetonitrile in water (with 0.1% formic acid) for 10 min and then separated on a Pepman100 RSLC C18 column (2.0 m, 75-m 150-mm) using a gradient of 2%40% acetonitrile with 0.1% formic acid over 120 min at a flow rate of 300 nL/min and a column temperature of 40C