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Summary of the factors involved in Cordyceps -induced anti-fatigue and antidepressant activity is depicted in Table 3

Importantly, commonly used in vitro BBB models, such as transwell systems and endothelial cell lines including RBE4, bEnd.3, and hCMEC/D3 monolayers, have important limitations, as they may overpredict permeability and do not fully recapitulate key in vivo BBB features, including physiological shear stress and multicellular neurovascular architecture (Chaulagain et al., 2023)

This has implications for the genetic diagnosis of THAP1 dystonia and supports the use of broader strategies like targeted next generation or whole exome sequencing in this population

The resulting glutamine is subsequently transported to neurons, where it is converted back into glutamate by glutaminase, providing a continuous supply of this key excitatory neurotransmitter 35,71