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Once the proteins are attached to the stationary phase, they can be separated by gradually reducing the salt concentration

2-chloro- N -phenylacetamide, 4-bromo-2-methoxy- N -phenylbenzamide, 3-fluoroacetanilide, N -methylacetanilide and 4-nitroacetanilide were purchased from Fluorochem (Hadfield, UK)

In some experiments, Vig (1 mM), GABA (0.1 M~1 mM), NV118 (25 M), GABA A -R antagonists including bicuculline (Bicl, 5 or 50 M), picrotoxin (PicroT, 5 or 50 M) and flumazenil (10 or 1 M), R162 (20 M), oligomycin (1.5 M), FCCP (1 M), or AG (0.2 mM) was added to cell culture medium

Evala 55 vitaminas, minerales y aminocidos