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Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans

Cuprizone-brain damage and motoric disability Cuprizone 2.5% of diet regimen was combined with BPC 157 in drinking water 10 g/kg or 10 ng/kg, 0.16 g/ml/12 ml/day or 0.16 ng/ml/12 ml/day till the sacrifice after four days, with the additional applications once daily, last application at 24 hours before sacrifice: cuprizone 1 g/kg intragastrically while BPC 157 was given 10 g/kg or 10 ng/kg intragastrically, controls received intragastrically an equivolume (5 ml/kg) of saline

This lysis and solubilization buffer was called LSB-2

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