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Cells were spun down at 3000 g for 5 min, the supernatant was discarded, the pellet was resuspended in 30 mL cold H 2 O and spun down as before described, supernatant was discarded
AA sensing and metabolic adaptation are particularly relevant in cancer, where specific AAs become metabolic dependencies
M., Cusi, K., Charlton, M., & Sanyal, A
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