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Determination of reactive oxygen species (ROS) ROS quantitation in brain tissue homogenates was measured using the 2,7-dichlorofluorescein diacetate (DCFH-DA) assay, as illustrated previously 68

Western blotting of the bladder samples from the test group revealed lesser expression of NF-B and subsequent reduced production of Cox-2 and other proteins involved in inflammation, showing marked anti-inflammatory activity of lactone parthenolide in IC (Kiuchi et al., 2024)

Its worth noting that excess B12 blood serum levels can come from taking the common, cheap form of B12, cyanocobalamin, which for many does not convert into an active form the body needs to absorb and use - therefore it does not get transported for cellular use (and is instead floating around in the bloodstream without positive effect)