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The supernatant (150 L) was placed in a clear flat-bottom 96-well plate in duplicate and absorbance measured at 620 nm in a Biotek Synergy HT plate reader

Peer-review started: June 6, 2017 First decision: June 22, 2017 Article in press: November 28, 2017 P- Reviewer: Bourgoin SG, Fujita T S- Editor: Gong ZM L- Editor: A E- Editor: Ma YJ Contributor Information Antonija Duzel, Departments of Pharmacology and Pathology, Medical Faculty University of Zagreb, Zagreb 10000, Croatia