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At the same time, liposomal nanoparticles LNP-CDN loaded with cyclic dinucleotides (CDN) can not only protect CDN from enzymatic degradation in malignant pleural effusion (MPE) but also specifically target macrophages and DCs, in addition, LNP-CDN alone or in combination with anti-PD-L1 antibody can not only lead to significant up-regulation of M1-related genes in TAMs, promote NK cells maturation and enhance the cytotoxic activity of NK cells, but also significantly increase the number of CD8 + T cells and reduce the volume of MPE, inhibit tumor growth, and ultimately significantly prolonging the survival of MPE tumor-bearing mouse (222)

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These proinflammatory pathways mainly culminate in the induction of several proinflammatory cytokines, such as TNF, IL1, IL6, and interferons (IFNs), as well as thymic stromal lymphopoietin (TSLP) and chemokines, including IL8, CXCL1, CXCL2, CXCL10, CCL2, and CCL20

Finally, the Aperio system (Leica) was used to observe the slices, in which 5 typical areas were randomly selected to manually count the number of positive cells (400 times magnification)