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Description
Pmc3391239

sgRNA complexes were synthesized by mixing equal volumes of crRNA (2 l, 160 M in nuclease-free water) and tracrRNA (2 l, 160 M) and incubated at 37 C for 30 min before Cas9 (2.7 l, 10 g l 1

Tibes R, Meurice N, Anthony SP, Qiu Y, Kornblau SM , Mousses S, Petit J

28 Improved NF-B signaling drives the expression of target genes, including mucin 5AC, oligomeric mucus/gel-forming (MUC5AC) that is known worsening COPD pathogenesis via inducing mucus obstruction
