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The number of tadpoles collected varied at different stages according to the weight of the samples in order to quantify total proteins and to standardize and dosage of biochemical biomarkers
We found that metabolic flux was geared towards glucose homeostasis regardless of the glucose dose

J Proteome Res 14:42074222 Couto N, Wood J, Barber J (2016) The role of glutathione reductase and related enzymes on cellular redox homoeostasis network

If the bag was in a car or near a heat source, use the danger zone assessment criteria above