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456 Altered energy substrate (e.g., glucose, lipid, and glutamate) metabolism is commonly found in the stressed cells

Annual Review of Nutrition

Most improvements of therapy (e.g., alpha-agonists) aim to selectively (eg, sub-type-selective alpha1-adrenoceptor agonists) affect urethral pressure, peripherally [5], and most recently, centrally [6], and these therapies may affect the bladder or urethral properties of healthy subjects (e.g., selective, partial agonist at the human alpha1(A)-adrenoceptor) [6], explaining the elevation in leak point pressure (LPP)

Intracellular calcium concentration was measured by flow cytometry using a Fluo-4 AM fluorescent probe (Beyotime, Cat# S1061S, Shanghai, CN)