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We recommend 35 mL of bacteriostatic water, though anywhere between 1 mL and 3 mL is acceptable

Cryoprotectant agent toxicity in porcine articular chondrocytes
And stay with me until the end, because I'm going to break down which GLP ones are typically used in this scenario

The authors concluded that cyclopentanone had no effect on fertility in mice (Hall et al., 1974)
