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This 83-amino-acid polypeptide features a key structural modification: a substitution of arginine for glutamic acid at position 3, along with a 13-amino-acid N-terminal extension

To enhance myelin purity, crude myelin was resuspended in 0.32 M sucrose buffer and submitted to a second sucrose gradient centrifugation followed by 2 repeated osmotic shocks in hypotonic Tris-HCl buffer as described above

Linus Pauling suggested decades ago that taking large doses of Vitamin C (megadosing) has an important role to play in human health, specifically in reducing the duration and severity of colds and even more serious and chronic health conditions (1)

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