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5 sessions dramatically outperformed 3 sessions (p = 0.0025) Compared to the same group's earlier 3-session protocol, the 5-session approach produced significantly better outcomes: median TSAR of 26.5% versus 10% with 3 sessions (Mann-Whitney U test, p = 0.0025)

In this study, we constructed a benchmark dataset from publicly available resources by merging peptide records and removing duplicate sequences, resulting in 426 positive and 6,865 negative samples