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PERK and GCN2 phosphorylation were not significantly increased with 4CBA treatment, allowing us to identify PKR as the main kinase participating in the integrated stress response triggered by CoQ deficiency

Bioinformatics Prediction Analysis In Silico Functional Prediction Tools Functional predictions were established from sequence homology based on different in silico programs, namely, Mutation Taster ( SIFT (Sorting Intolerant From Tolerant) ( and PolyPhen-2 (Polymorphism Phenotyping v2) ( Both of the SIFT and PolyPhen-2 tools report results in terms of pathogenic scores as benign, tolerated, or deleterious, whereas Mutation Taster scores classified the detected variants to be disease causing or neutral

Thus, we employed LDTS-LIR mut as a negative control to evaluate the effect of lipophagy activation with the LDTS-eLIR construct

Collier, T