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Glutathione and Nitrosoglutathione in Macrophage Defense Against Mycobacterium Tuberculosis

While the pro-inflammatory effect of microcrystalline UA is well-established 3,20 , there are several indications that soluble UA free of UA crystals has protective effects against inflammatory mechanisms and toxicity 50 with effects on macrophage polarisation towards the anti-inflammatory M2 phenotype 51

doi: 10.1038/s41583-023-00724-7 43 ZhuAHuangJKongFTanJLeiJYuanYet al

Techniques used include: ESI-MS (electrospray ionization) MALDI-TOF (laser ionization) Storage and laboratory handling Storage Conditions Correct storage is crucial to maintaining the integrity and activity of the peptide: Freeze-dried form (unopened): Temperature: -20C (optimal) or 2-8C (short-term allowable) Protect from light and moisture Store in the original packaging Shelf life: 24 months from the production date at -20C After reconstitution: Store at 2-8C Use within 7-14 days For longer storage: freeze at -20C in small portions (aliquots) Avoid repeated freezing and thawing Reconstitution Instructions Before opening, leave the vial at room temperature for 15-30 minutes Use sterile bacteriostatic water or appropriate buffer (e.g