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Earlier reports reveled that p53 plays a crucial role in regulating G1 and G2 phases of the cell cycle 75 and mediates transcriptional activation of Bax 76,77

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Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

B12 is an essential water-soluble vitamin that helps the body with numerous functions