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Main takeaway: Use GHK-Cu Face Serum after cleansing and before face cream or moisturiser

Data was analysed by repeated measures one-way ANOVA with Tukey correction for multiple comparisons

Existing solutions include developing multi-ligand collaborative targeting to enhance binding efficiency and using low immunogenic ligands to reduce immune responses

In vitro cytotoxicity test Epidermal culture To investigate the safety performance of prepared hydrogels for further use in clinical trials, cytotoxicity evaluation was conducted using Epiderm Assay Kit, i.e., EpiDerm (EPI-200, MatTek Corporation, Ashland, MA, USA), which is commercially available human epidermal equivalent