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Data derived from the mouse model of type 2 inflammation also showed that total ILC2s and TLR4 + ILC2s in the lungs of these animals were increased from baseline levels following both specific and non-specific allergen challenge, and that IL-13 + TLR4 + ILC2s remained at a high level even four weeks following cessation of allergen challenge

In addition, the lack of an endogenous equivalent of the HaloTag protein in mammalian cells minimizes the chance of detecting false positives or nonspecific interactions

Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific)

Factor 2: Your lowest dose must be measurable on your syringe