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Harth G, Horwitz MA: Inhibition of Mycobacterium tuberculosis glutamine synthetase as a novel antibiotic strategy against tuberculosis: demonstration of efficacy in vivo

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RNA isolation and qRT-PCR RNA was isolated using TRIreagent (Merck) and contaminating genomic DNA was removed with the RapidOut DNA removal kit (Thermo Fisher Scientific)

By encouraging the development of IL-1 and IL-18 in a caspase-1-dependent manner, this activation intensifies neuroinflammatory cascades and fosters an environment that is antagonistic to dopaminergic neurons [60, 61]