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Immunofluorescence confocal microscopy After the indicated treatment, EGFP-NINJ1-overexpressing HT-1080 cells, with or without mCherry-xCT or TFRC-mApple overexpression, were fixed with 3.7% formaldehyde, permeabilized using 0.3% Triton X-100, and blocked with 5% BSA for 1 h
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