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qPCR measurements were performed in triplicates with MaximaTM SYBR Green/ROX qPCR Master Mix (Thermo Scientific) in an ABI 7300 Real-time PCR machine using the Comparative Cycle Threshold Method

PD-L1 induces ERK-dependent CEBPB phosphorylation, activating BAG-1 transcription and reinforcing TKI resistance [140]

However, its high cost limits access in some social strata, therefore, other methods are used, such as: abrasion with diamond cutters, cryosurgery and electrosurgery
Transcriptomic analysis showed increase in mitochondrial biogenesis, and oxidative phosphorylation