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The method was adapted to allow for experimental measurements where the same readout was observed for multiple different test material treatments and concentrations, observed at different timepoints (Middleton et al., in preparation)

10.1016/j.jep.2015.02.026 Summary Keywords therapeutic polysaccharides of natural origin, type 2 diabetes mellitus, structural characterization, complications of type 2 diabetes mellitus, mechanism Citation Liu Z, Wang H, Gong X, Jiang X, Yang K, Jiang L, Wang Z and Tong Q (2025) Recent advances in natural polysaccharides for type 2 diabetes management: sources, structural characteristics, and mechanistic insights

This compromised mitochondrial function is driven by the combination of a shortfall in glutathione precursor amino acids (cysteine and glycine) and the accumulation of homocysteine (a toxic transsulfuration/glutathione biosynthesis pathway intermediate) [93]

This is achieved by being a substrate of phosphofructokinase-2, forming fructose-2,6-bisphosphate