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The cDNA was subjected to PCR with Taq (Takara), dNTPs (TOYOBO), and following primers: GS (GLUL) , 5-CGAAGTATTCAAGTATAACCGGAAG-3 and 5-TCACCATGTCCATTATAC GCTTAC-3

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A list of all authentic reference standards corresponding to endogenous BAs used to confirm BA identities are listed in Supplementary Table S1

AMA Fasting: benefits for body composition and disease prevention, potential risks, and Peters updated practical framework #353 AMA #72: Fasting: benefits for body composition and disease prevention, potential risks, and Peters updated practical framework In this Ask Me Anything (AMA) episode, Peter revisits the topic of fasting, sharing how his thinking has evolved over time