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10.1007/s00737-022-01220-0 38

This indicates a disruption in estrogen homeostasis, which is not only associated with reproductive system abnormalities, such as precocious puberty, but may also lead to impaired cognitive function

The coding region was cloned into a modified pETM-11 bacterial expression vector which was derived from a pET-24d(+) vector (Novagen) by insertion of a tobacco etch virus (TEV) protease cleavage site following an N terminal hexa-histidine and protein A tag