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Rat kidney sections of MTX self treated showed severe lose of kidney architecture, marked disturbance in glomeruli seemed to have lost their attachments and mesangial stroma and others were atrophied with dilatation in the sub capsular space (Figure 2F)

Sterol analysis was done as previously described 49

It duplicated the previous frame

Conversely a decrease in cellular cholesterol levels will increase SREBP2 expression resulting in an increase in LDL receptor activity and an increase in miR-33, which will result in a decrease in LXR activity, decreased expression of ABCA1 and ABCG1, and a reduction in cholesterol efflux