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In the experiments with mitochondria, O 2 consumption was measured directly, and dissociation and reduction of cyt c from the membrane could not be monitored

Acetylcholine esterase assay The quality of the isolated exosomes was further verified by measuring acetylcholinesterase activity using the validated Amplex TM Red Acetylcholine/Acetylcholinesterase Assay Kit (Molecular Probes, Invitrogen)