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Proteins were detected with the indicated antibodies listed in the Reagents and Tools table, and blots were imaged using Odyssey Imaging System

20 The toxins share multiple conserved domains including a catalytic glucosyltransferase domain located at the N-terminus and a cysteine protease domain which is required for auto-processing and delivery of the catalytic domain to the cytosol of the intoxicated cell

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It donates methyl groups to synthesize creatine, carnitine, and phosphatidylcholine while also serving as a precursor to glutathione, the body's primary intracellular antioxidant