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This includes regulatory T (Treg) cells, myeloid-derived suppressor cells, tumor-associated macrophages, and increased expression of various immunosuppressive molecules, such as PD-1 and PD-L1

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Even large intranuclear vacuoles are mostly undetectable with optical magnifications used in conventional ICSI but they do appear at the magnification used in IMSI (Figure 2B) so that injection of spermatozoa with such vacuoles into oocytes can be avoided [191,193]