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Reduction of total E2F/DP activity induces senescence-like cell cycle arrest in cancer cells lacking functional PRB and p53

doi: 10.1161/01.RES.87.5.349 [DOI] [PubMed] [Google Scholar] [116].Nagaoka S-i, Kakiuchi T, Ohara K, et al

The seeding density was set at 1 10 4 cells per well, and the plate was incubated for a period of 24 h in RPMI culture medium containing 10% FBS and 1%penicillinstreptomycin in a 37 C incubator with 5% CO 2 and appropriate humidity to allow for cell attachment and growth

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