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BMJ Open 12: e063520, 2022

First, 500 l of a 30% iodixanol (Optiprep solution, D1556, Merck) prepared in a buffer containing 0.32 M sucrose, 6 mM EDTA, and 60 mM HEPES-NaOH (pH 7.4) was dispensed into a centrifuge tube

The consequent ROS accumulation in the body is inevitably associated with tissue injury [1], since ROS are known to damage cell components, particularly DNA, leading to loss of function, induction of apoptosis, or even carcinogenesis [2]

Vitamin B12 (as Methylcobalamin & Hydroxycobalamin) This vitamin is required for red blood cell formation, DNA synthesis, and proper neurological function and repair due to its critical role in myelin sheath health and regeneration