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This model proposes that mGSH depletion due to its covalent binding to NAPQI acts as a first-hit, which is followed by a burst of mitochondrial ROS production that activates a second hit involving mitogen-activated protein kinases (MAPKs) that in turn amplifies the wave of mitochondrial ROS generation (Lemasters, 1998)
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Trabecular bone in the distal femur was analyzed beginning 10 slices away from the growth plate to exclude the primary spongiosa, extending for a total of 150 slices
