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Considerations High Concentration Effects When the concentration of a solution is too high, the solution may experience light scattering, or the absorbance may exceed the instruments linear range, leading to measurement errors

doi: 10.1515/med-2016-0072 149 YangY.LiuP

Furthermore, a lot of trials are underpowered because of their short durations or small sample numbers, which restricts their statistical reliability and generalizability to larger populations (74)