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Role of Melanin in Skin Darkening Melanin is the pigment that gives our skin, hair, and eyes their color

Importantly, the so-called Hook effect, also known as the prozone effect, antibody excess, or the prozone phenomenon, occurs when the target antigen/antibody is in greater concentrations than those of the capture antibodies and vice versa (i.e., when the capture antibodies are very low levels compared to the target), and it can trigger false-negative test results in several assay techniques, including one-step immunometric assays, TIAs, NMAs, ELISAs, LFIAs, and other immunochromatographic assays [1156,1160,1189,1190,1191]

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