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The interaction of TJ proteins with the actin cytoskeleton is vital to the maintenance of TJ structure and permits the cytoskeletal regulation of TJ barrier integrity.17 The circumferential contraction and tension in the actin is regulated by myosin light chain (MLC) activation.18 Induction of MLC phosphorylation by kinases such as MLC kinase (MLCK) and Rho-associated kinase causes the contraction of the actin, resulting in the opening of the paracellular pathways.18,19 1

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