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Expression of estrogen receptor (ER) subtypes and ERbeta isoforms in colon cancer

Each PCR product was analyzed by Sanger sequencing with sequencing primer SI#658, and sequencing traces were processed using PySanger ( Canavanine assay Genome editing efficiencies of different Cas9-based genome editing enzymes (Cas9, dCas9, dCas9-PmCDA1, dCas9-PmCDA1-UGI, nCas9, nCas9-PmCDA1 and nCas9-PmCDA1-UGI) were estimated using a canavanine assay