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Description
The method was described in a previous report 20

Kurpad et al

Pharmacol Res 41 : 341346 Kuratsune H, Watanabe Y, Yamaguti K, Jacobsson G, Takahashi M, Machii T, Onoe H, Onoe K, Matsumura K, Valind S, Kitani T, Lngstrm B

The UPR consists of three main signaling pathways, PERK, IRE-1, and ATF6., that normally bound by a chaperone called Grp78 and are inactive
