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High-Salt-Diet (HSD) aggravates the progression of Inflammatory Bowel Disease (IBD) via regulating epithelial necroptosis

Most importantly, design studies to answer mechanistic questions (does the peptide activate the expected pathway?) before efficacy questions (does it improve venous function?)

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enzymolysis Protein extract was treated with 10mM DTT at 95 C for 3min and alkylated using 25 mM iodoacetamide at room temperature for 30min while being protected from light and subjected to clean up and digestion with single-pot solid phase-enhanced sample preparation (SP3) beads